Journal: Biology

Article Title: The Effects of Aldosterone on Hypertension-Associated Kidney Injury in a Tg-hAS Mouse Model

doi: 10.3390/biology13121084

Figure Lengend Snippet: The uACR in Tg-hAS mice treated with high salt in the presence or absence of FAD286 or spironolactone. The 1-month HS-fed female WT and Tg-hAS transgenic mice were treated with or without FAD286 or SPL for 2 months, and the uACRs of mice were measured. ( A ) The percentage of mice with a uACR > 30 mg/g ( B ). Data are presented as the mean ± SD, n = 5–10, ** p < 0.01. FAD: FAD286; SPL: spironolactone; WT: wild type; hAS: Tg-hAS; NS: normal salt; HS: high salt. ACR: urine-albumin-to-creatinine ratio.

Article Snippet: Albumin level in urine was measured using mouse albumin ELISA kit (ab108792, Abcam, Cambridge, MA, USA), and urine creatinine level was measured using creatinine parameter assay kit (KGE005, R&D systems, Minneapolis, MN, USA). uACR was calculated to detect kidney injury.

Techniques: Transgenic Assay

Journal: Experimental & Molecular Medicine

Article Title: Dynamics of chromatin accessibility governing Gd-IgA1 synthesis in B cells associated with IgA nephropathy

doi: 10.1038/s12276-025-01505-1

Figure Lengend Snippet: a Transfection of the DAKIKI cell line with adenoviruses containing coding sequences for KLF4, followed by western blot analysis to assess target protein expression. b , c Collection of culture supernatant 3 days after transfection for IgA1 ( b ) and Gd-gA1 ( c ) ELISA analysis ( n = 6). d Transfection of EBV-immortalized B cells with adenoviruses containing coding sequences for KLF4, followed by western blot analysis for target protein expression. e , f Collection of culture supernatant from EBV-immortalized B cells 3 days after transfection for IgA1 ( e ) and Gd-gA1 ( f ) ELISA analysis ( n = 6). g Electroporation of synthetic siRNA targeting the human KLF4 gene or control siRNA into the DAKIKI cell line. h , i Collection of culture supernatant from the DAKIKI cell line 3 days after electroporation for IgA1 ( h ) and Gd-gA1 ( i ) ELISA analysis ( n = 6). j , Electroporation of synthetic siRNA targeting the human KLF4 gene or control siRNA into the EBV-immortalized B cell lines. k , l Collection of culture supernatant from EBV-immortalized B cell lines 3 days after electroporation for IgA1 ( k ) and Gd-gA1 ( j ) ELISA analysis ( n = 6). Bars represent the mean ± s.e.m. * P < 0.05, ** P < 0.01.

Article Snippet: Spot-urine samples were collected, and the levels of urinary albumin and creatinine were measured using urinary albumin ELISA kits (Elabscience) and creatinine ELISA kits (Elabscience), respectively.

Techniques: Transfection, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Electroporation, Control

Journal: Immunologic Research

Article Title: MYC affects mitochondrial function in IgA nephropathy by promoting the degradation of MFN1 through HRD1

doi: 10.1007/s12026-025-09695-6

Figure Lengend Snippet: Down-regulation of HRD1 alleviates renal injury in IgAN. A Changes in HRD1 expression levels after HRD1 down-regulation in IgAN mice. B Changes in HRD1 protein expression levels after HRD1 down-regulation in IgAN mice. C Changes in IgA expression levels after HRD1 knockdown in IgAN mice. D HE staining was used to detect the pathological changes in renal tissues. E Masson staining was employed to evaluate collagen fiber deposition. F PAS staining was used to detect the lesions in glomeruli and tubules. G TUNEL was used to assess cell apoptosis after knockdown of HRD1 in IgAN mice. H Changes in the levels of proteinuria, creatinine and BUN in IgAN mice with HRD1 deficiency. The image above is taken at 200 × magnification, and the image below is taken at 400 × magnification (Figs. 2D-F). * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bars: 50 μm (A, C, D, E, and F)

Article Snippet: Human IL-6 ELISA kit (Elabscience, E-EL-H6156), Human IL-1β ELISA kit (Elabscience, E-EL-H0149), Human TNF-α ELISA kit (Elabscience, E-EL-H0109), Urine protein Assay Kit (Nanjing Jiancheng Bioengineering Institute, C035-2–1), Creatinine Assay Kit (Nanjing Jiancheng Bioengineering Institute, C011-2–1), Urea Assay Kit (Nanjing Jiancheng Bioengineering Institute, C013-2–1), ATPase assay kit (Nanjing Jiancheng Bioengineering Institute, A070-1).

Techniques: Expressing, Knockdown, Staining, TUNEL Assay